valuation of antispasmodic effect of exracts and essential oil of Thymus daenensis L. on rat uterus contractions

Document Type : Research paper

Authors

1 1Associate Professor, Dept. of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Assistant Professor, Dept. of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Ph.D, Dept. of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Thymus daenensis L. is a local species of Lamiaceae family found in Iran. T. daenensis traditionally has been used by local people. The aim of this research was to assess spasmolytic action of T. daenensis hydroalcoholic extract, hexane partition and the essential oil on uterus contraction. For pharmacological studies, isolated rat uterus suspended in an organ bath filled by Tyrode’s solution, was contracted by KCl, acetylcholine or oxytocin. Inhibitory action of T. daenensis extracts and essential oil was examined on above spasmogens in rat uterus and recorded on oscillograph apparatus. Nifedipine was used as standard drugs. Nifedipine in a concentration manner inhibited rat uterus contractions induced by KCl, acetylcholine and oxytocin. The hydroalcoholic extract (20µg/ml-1.28mg/ml), hexane partition and essential oil of T. daenensis also attenuated uterine contractions induced by above spasmogens in the organ bath. Comparing the inhibitory effect of hydroalcoholic extract with hexane partition indicate that the hexane partition contains more potent components. The essential oil of T. daenensis was even more potent than the hexane partition. Therefore, it looks like that the lipophilic constituents in the hexane partition and the essential oil are mainly responsible for inhibitory action of T. daenensis.

Keywords

References

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Advanced Research in Medicinal Plants
Volume 2, Issue 3 - Serial Number 4
November 2025
Pages 33-43

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History

  • Receive Date: 15 March 2025
  • Revise Date: 12 July 2025
  • Accept Date: 27 September 2025

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