The effect of hydroalcoholic extract of cumin and ginger plants separately and ‎combined on MDA-MB-231 and MCF-7 breast cancer cell lines

Document Type : Research paper

Authors

1 Assistant Professor, Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

2 M.Sc., Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

This study investigates the toxicity effect of cumin and ginger extracts on breast ‎cancer cell lines MDA-MB-231 and MCF-7 and compare them with RHDP and Vero as ‎normal cells. The extracts of two plants were added to the medium of these 4 cell lines and after 72 h, cell viability was measured by the MTT method. The cumulative ‎effect of two extracts with a concentration of 100 μg/ml of cumin and different ‎concentrations of ginger extract was investigated. AnnexinV-FITC/PI staining was carried out ‎to determine the level of apoptosis.‎ In MDA cells, the IC50 of cumin and ginger were 107.4 µg/ml and 158.8 µg/ml, and ‎in the MCF-7 cell line, the IC50 of cumin and ginger were 197.4 µg/ml and ‎‎120.4 µg/ml, respectively. Ginger and cumin extracts in VERO and RHDP cells increased ‎the survival rate of these cells, up to 200 µg/ml. The IC50 in these cells was around 700 µg/ml ‎or higher. The combined use of these two extracts decreased the IC50 ‎concentration of both plants. In AnnexinV-FITC/PI staining, a total of about ‎‎28 % of the cells under the influence of two extracts became apoptotic after 36 ‎h (vs 10.5 % in control). Therefore, cumin and ginger extracts in ‎harmless titers for normal cells can inhibit the proliferation of cancerous ‎cells.‎

Keywords

References

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Advanced Research in Medicinal Plants
Volume 1, Issue 1 - Serial Number 1
December 2022
Pages 37-48

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History

  • Receive Date: 25 October 2022
  • Revise Date: 31 December 2022
  • Accept Date: 08 January 2023

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