Effect of different extraction methods on the metabolites and biological activities of chaville seeds (Ferulago angulate)

Document Type : Research paper

Authors

1 Assistant Professor, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran

2 M.Sc. Student, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran

3 Associate Professor, Department of Agronomy, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran

Abstract

Chaville (Ferulago angulate) belonging to the Apiaceae family, is a native medicinal plant which is distributed in the west and south west regions of Iran. It is used as a sedative and digestive drug since ancient time and is consumed to treat intestinal worms, antioxidants, antimicrobials, anticancer drugs, blood pressure treatments, and Alzheimer’s and diabetes effects. In this study, the effects of different extraction methods including ultrasound, agitation, maceration, soxhlet, reflux and microwave were investigated on the content of phenolic compounds and antioxidant activity of methanolic extract. Determination of total phenol and flavonoid contents were determined using spectrophotometric methods and antioxidant activity of the extracts were also assayed by DPPH method. According to the results, the extract obtained by soxhlet method had the highest yield. Also, the highest total phenol content (26.77 mg GAE/ g extract) belonged to the agitation method, while the highest total flavonoid content (5.82 mg QE/ g extract) was reported by the soxhlet method. The highest antioxidant activity was achieved at the extract obtained by soxhlet and reflux methods with IC50 = 59 μg/mL. The results indicated that extraction methods play an important role in the quality and antioxidant activity of active compounds. In conclusion, soxhlet method was the best method in order to increase the yield of active compounds at Ferulago angulate.

Keywords

References

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Advanced Research in Medicinal Plants
Volume 1, Issue 1 - Serial Number 1
December 2022
Pages 1-10

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History

  • Receive Date: 14 May 2022
  • Revise Date: 08 October 2022
  • Accept Date: 08 October 2022

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